Loss of miR-542-3p enhances IGFBP-1 expression in decidualizing human endometrial stromal cells.

ABSTRACT: Endometrial decidualization represents an essential step for the successful implantation of the embryo; however, the molecular mechanism behind this differentiation process remains unclear. This study aimed to identify novel microRNAs (miRNAs) involved in the regulation of decidual gene expression in human endometrial stromal cells (HESCs). An in vitro analysis of primary undifferentiated and decidualizing HESCs was conducted. HESCs were isolated from hysterectomy specimens from normally cycling premenopausal women with uterine fibroids, who were not on hormonal treatment at the time of surgery. Primary HESCs were expanded in culture and decidualized with 8-bromo-cyclic adenosine monophosphate and medroxyprogesterone acetate. Microarray analysis identified six miRNAs differentially express

SUBMITTER: Tochigi H 

PROVIDER: S-EPMC5209665 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature