Sevoflurane binds and allosterically blocks integrin lymphocyte function-associated antigen-1.

ABSTRACT:

Background

Volatile anesthetics have been shown to modify immune cell functions via several mechanisms, some of which have been only partially elucidated. We demonstrated that isoflurane inhibits primary leukocyte integrin lymphocyte function-associated antigen-1 (LFA-1) by binding to the allosteric cavity critical for conformational activation to its high-affinity form. It remains to be determined whether the allosteric inhibition of LFA-1 by isoflurane can be generalized to other anesthetics such as sevoflurane.

Methods

The effects of sevoflurane on the ability of LFA-1 to bind to its counter-ligand, intercellular adhesion molecule-1, was studied in leukocytes by flow cytometry. To examine whether sevoflurane acts directly on LFA-1, we measured ligand-binding using beads c

SUBMITTER: Yuki K 

PROVIDER: S-EPMC5209785 | biostudies-literature | 2010 Sep

REPOSITORIES: biostudies-literature