A molecular genetic toolbox for Yarrowia lipolytica.

ABSTRACT:

Background

Yarrowia lipolytica is an ascomycete yeast used in biotechnological research for its abilities to secrete high concentrations of proteins and accumulate lipids. Genetic tools have been made in a variety of backgrounds with varying similarity to a comprehensively sequenced strain.

Results

We have developed a set of genetic and molecular tools in order to expand capabilities of Y. lipolytica for both biological research and industrial bioengineering applications. In this work, we generated a set of isogenic auxotrophic strains with decreased non-homologous end joining for targeted DNA incorporation. Genome sequencing, assembly, and annotation of this genetic background uncovers previously unidentified genes in Y. lipolytica. To complement these

SUBMITTER: Bredeweg EL 

PROVIDER: S-EPMC5210315 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature