A high-resolution radiation hybrid map of chicken chromosome 5 and comparison with human chromosomes.

ABSTRACT:

Background

The resolution of radiation hybrid (RH) maps is intermediate between that of the genetic and BAC (Bacterial Artificial Chromosome) contig maps. Moreover, once framework RH maps of a genome have been constructed, a quick location of markers by simple PCR on the RH panel is possible. The chicken ChickRH6 panel recently produced was used here to construct a high resolution RH map of chicken GGA5. To confirm the validity of the map and to provide valuable comparative mapping information, both markers from the genetic map and a high number of ESTs (Expressed Sequence Tags) were used. Finally, this RH map was used for testing the accuracy of the chicken genome assembly for chromosome 5.

Results

A total of 169 markers (21 microsatellites and 148 ESTs) were typed on the C

SUBMITTER: Pitel F 

PROVIDER: S-EPMC521070 | biostudies-literature | 2004 Sep

REPOSITORIES: biostudies-literature