Role of the BrafV637E mutation in hepatocarcinogenesis induced by treatment with diethylnitrosamine in neonatal B6C3F1 mice.

ABSTRACT: The BrafV637E mutation is frequently reported in mouse hepatic tumors, depending on the mouse strain, and corresponds to the human BrafV600E mutation. In this study, we detected the BrafV637E mutation by whole-exome analysis in 4/4 hepatic tumors induced by neonatal treatment with diethylnitrosamine (DEN) in male B6C3F1 mice. We also detected the BrafV637E mutation in 54/63 (85.7%) hepatic lesions, including microscopic foci and grossly visible tumors, by PCR-direct sequencing. Although the mutation was detected in 5/7 (71.4%) hepatic tumors induced by neonatal DEN treatment followed by repeated CCl₄ administration, it was not detected in 24 tumors induced by CCl₄ treatment without DEN or in eight spontaneous lesions in B6C3F1 mice, suggesting that the mutation is ind

SUBMITTER: Yamamoto M 

PROVIDER: S-EPMC5214095 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature