Dissecting the genetic components of a quantitative trait locus for blood pressure and renal pathology on rat chromosome 3.

ABSTRACT:

Background

We have previously confirmed the importance of rat chromosome 3 (RNO3) genetic loci on blood pressure elevation, pulse pressure (PP) variability and renal pathology during salt challenge in the stroke-prone spontaneously hypertensive (SHRSP) rat. The aims of this study were to generate a panel of RNO3 congenic sub-strains to genetically dissect the implicated loci and identify positional candidate genes by microarray expression profiling and analysis of next-generation sequencing data.

Method and results

A panel of congenic sub-strains were generated containing Wistar-Kyoto (WKY)-introgressed segments of varying size on the SHRSP genetic background, focused within the first 50 Mbp of RNO3. Haemodynamic profiling during salt challenge demonstrated significantly red

SUBMITTER: Koh-Tan HH 

PROVIDER: S-EPMC5214373 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature