Dynamic Changes in Chromatin Accessibility Occur in CD8⁺ T Cells Responding to Viral Infection.

ABSTRACT: In response to acute infection, naive CD8⁺ T cells expand, differentiate into effector cells, and then contract to a long-lived pool of memory cells after pathogen clearance. During chronic infections or in tumors, CD8⁺ T cells acquire an "exhausted" phenotype. Here we present genome-wide comparisons of chromatin accessibility and gene expression from endogenous CD8⁺ T cells responding to acute and chronic viral infection using ATAC-seq and RNA-seq techniques. Acquisition of effector, memory, or exhausted phenotypes was associated with stable changes in chromatin accessibility away from the naive T cell state. Regions differentially accessible between functional subsets in vivo were enriched for binding sites of transcription factors known to regulate these

SUBMITTER: Scott-Browne JP 

PROVIDER: S-EPMC5214519 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature