Metabolomic Profiling in Individuals with a Failing Kidney Allograft.

ABSTRACT:

Background

Alteration of certain metabolites may play a role in the pathophysiology of renal allograft disease.

Methods

To explore metabolomic abnormalities in individuals with a failing kidney allograft, we analyzed by liquid chromatography-mass spectrometry (LC-MS/MS; for ex vivo profiling of serum and urine) and two dimensional correlated spectroscopy (2D COSY; for in vivo study of the kidney graft) 40 subjects with varying degrees of chronic allograft dysfunction stratified by tertiles of glomerular filtration rate (GFR; T1, T2, T3). Ten healthy non-allograft individuals were chosen as controls.

Results

LC-MS/MS analysis revealed a dose-response association between GFR and serum concentration of tryptophan, glutamine, dimethylarginine isomers (asymmetric [A]DMA an

SUBMITTER: Bassi R 

PROVIDER: S-EPMC5214547 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature