Recurrent Clostridium difficile infection associates with distinct bile acid and microbiome profiles.

ABSTRACT:

Background

The healthy microbiome protects against the development of Clostridium difficile infection (CDI), which typically develops following antibiotics. The microbiome metabolises primary to secondary bile acids, a process if disrupted by antibiotics, may be critical for the initiation of CDI.

Aim

To assess the levels of primary and secondary bile acids associated with CDI and associated microbial changes.

Methods

Stool and serum were collected from patients with (i) first CDI (fCDI), (ii) recurrent CDI (rCDI) and (iii) healthy controls. 16S rRNA sequencing and bile salt metabolomics were performed. Random forest regression models were constructed to predict disease status. PICRUSt analyses were used to test for associations between predicted bacterial bile salt h

SUBMITTER: Allegretti JR 

PROVIDER: S-EPMC5214573 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature