Honey Bee Deformed Wing Virus Structures Reveal that Conformational Changes Accompany Genome Release.

ABSTRACT: The picornavirus-like deformed wing virus (DWV) has been directly linked to colony collapse; however, little is known about the mechanisms of host attachment or entry for DWV or its molecular and structural details. Here we report the three-dimensional (3-D) structures of DWV capsids isolated from infected honey bees, including the immature procapsid, the genome-filled virion, the putative entry intermediate (A-particle), and the empty capsid that remains after genome release. The capsids are decorated by large spikes around the 5-fold vertices. The 5-fold spikes had an open flower-like conformation for the procapsid and genome-filled capsids, whereas the putative A-particle and empty capsids that had released the genome had a closed tube-like spike conformation. Between the two conformati

SUBMITTER: Organtini LJ 

PROVIDER: S-EPMC5215330 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature