Simultaneous high-speed imaging and optogenetic inhibition in the intact mouse brain.

ABSTRACT: Genetically encoded calcium indicators and optogenetic actuators can report and manipulate the activity of specific neuronal populations. However, applying imaging and optogenetics simultaneously has been difficult to establish in the mammalian brain, even though combining the techniques would provide a powerful approach to reveal the functional organization of neural circuits. Here, we developed a technique based on patterned two-photon illumination to allow fast scanless imaging of GCaMP6 signals in the intact mouse brain at the same time as single-photon optogenetic inhibition with Archaerhodopsin. Using combined imaging and electrophysiological recording, we demonstrate that single and short bursts of action potentials in pyramidal neurons can be detected in the scanless modality at ac

SUBMITTER: Bovetti S 

PROVIDER: S-EPMC5215385 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature