Regulation of alternative splicing in human obesity loci.

ABSTRACT:

Objective

Multiple obesity susceptibility loci have been identified by genome-wide association studies, yet the mechanisms by which these loci influence obesity remain unclear. Alternative splicing could contribute to obesity by regulating the transcriptomic and proteomic diversity of genes in these loci.

Methods

Based on a database search, 72 of the 136 genes at the 13 obesity loci encoded multiple protein isoforms. Thus, alternative splicing of these genes in adipose tissue samples was analyzed from the Metabolic Syndrome in Men population-based study and from two weight loss intervention studies (surgical and very low calorie diet).

Results

Alternative splicing was confirmed in 11 genes with PCR capillary electrophoresis in human subcutaneous adipose tissue. Intere

SUBMITTER: Kaminska D 

PROVIDER: S-EPMC5215786 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature