Ultra high performance liquid chromatography-high resolution mass spectrometry plasma lipidomics can distinguish between canine breeds despite uncontrolled environmental variability and non-standardized diets.

ABSTRACT:

Introduction and objectives

The purpose of this study was to use high accurate mass metabolomic profiling to investigate differences within a phenotypically diverse canine population, with breed-related morphological, physiological and behavioural differences. Previously, using a broad metabolite fingerprinting approach, lipids appear to dominate inter- and intra- breed discrimination. The purpose here was to use Ultra High Performance Liquid Chromatography-High Resolution Mass Spectrometry (UHPLC-HRMS) to identify in more detail, inter-breed signatures in plasma lipidomic profiles of home-based, client-owned dogs maintained on different diets and fed according to their owners' feeding regimens.

Methods

Nine dog breeds were recruited in this study (Beagle, Chihuahua, Cocker

SUBMITTER: Lloyd AJ 

PROVIDER: S-EPMC5216087 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature