Obtaining control of cell surface functionalizations via Pre-targeting and Supramolecular host guest interactions.

ABSTRACT: The use of mammalian cells for therapeutic applications is finding its way into modern medicine. However, modification or "training" of cells to make them suitable for a specific application remains complex. By envisioning a chemical toolbox that enables specific, but straight-forward and generic cellular functionalization, we investigated how membrane-receptor (pre)targeting could be combined with supramolecular host-guest interactions based on β-cyclodextrin (CD) and adamantane (Ad). The feasibility of this approach was studied in cells with membranous overexpression of the chemokine receptor 4 (CXCR4). By combining specific targeting of CXCR4, using an adamantane (Ad)-functionalized Ac-TZ14011 peptide (guest; K_D = 56 nM), with multivalent host molecules that entailed fluoresc

SUBMITTER: Rood MT 

PROVIDER: S-EPMC5216351 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature