Cyclin D1 unbalances the redox status controlling cell adhesion, migration, and drug resistance in myeloma cells.

ABSTRACT: The interactions of multiple myeloma (MM) cells with their microenvironment are crucial for pathogenesis. MM cells could interact differentially with their microenvironment depending on the type of cyclin D they express. We established several clones that constitutively express cyclin D1 from the parental RPMI8226 MM cell line and analyzed the impact of cyclin D1 expression on cell behavior. We performed a gene expression profiling study on cyclin D1-expressing vs. control cells and validated the results by semi-quantitative RT-PCR. The expression of cyclin D1 altered the transcription of genes that control adhesion and migration. We confirmed that cyclin D1 increases cell adhesion to stromal cells and fibronectin, stabilizes F-actin fibers, and enhances chemotaxis and inflammatory chemoki

SUBMITTER: Bustany S 

PROVIDER: S-EPMC5216717 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature