Hypoxia-mediated upregulation of MCT1 expression supports the glycolytic phenotype of glioblastomas.

ABSTRACT:

Background

Glioblastomas (GBM) present a high cellular heterogeneity with conspicuous necrotic regions associated with hypoxia, which is related to tumor aggressiveness. GBM tumors exhibit high glycolytic metabolism with increased lactate production that is extruded to the tumor microenvironment through monocarboxylate transporters (MCTs). While hypoxia-mediated regulation of MCT4 has been characterized, the role of MCT1 is still controversial. Thus, we aimed to understand the role of hypoxia in the regulation of MCT expression and function in GBM, MCT1 in particular.

Methods

Expression of hypoxia- and glycolytic-related markers, as well as MCT1 and MCT4 isoforms was assessed in in vitro and in vivo orthotopic glioma models, and also in human GBM tissues by immunofluorescenc

SUBMITTER: Miranda-Goncalves V 

PROVIDER: S-EPMC5216802 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature