ISeq: A New Double-Barcode Method for Detecting Dynamic Genetic Interactions in Yeast.

ABSTRACT: Systematic screens for genetic interactions are a cornerstone of both network and systems biology. However, most screens have been limited to characterizing interaction networks in a single environment. Moving beyond this static view of the cell requires a major technological advance to increase the throughput and ease of replication in these assays. Here, we introduce iSeq-a platform to build large double barcode libraries and rapidly assay genetic interactions across environments. We use iSeq in yeast to measure fitness in three conditions of nearly 400 clonal strains, representing 45 possible single or double gene deletions, including multiple replicate strains per genotype. We show that iSeq fitness and interaction scores are highly reproducible for the same clonal strain across replic

SUBMITTER: Jaffe M 

PROVIDER: S-EPMC5217104 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature