Time-Course Analysis of Gene Expression During the Saccharomyces cerevisiae Hypoxic Response.

ABSTRACT: Many cells experience hypoxia, or low oxygen, and respond by dramatically altering gene expression. In the yeast Saccharomyces cerevisiae, genes that respond are required for many oxygen-dependent cellular processes, such as respiration, biosynthesis, and redox regulation. To more fully characterize the global response to hypoxia, we exposed yeast to hypoxic conditions, extracted RNA at different times, and performed RNA sequencing (RNA-seq) analysis. Time-course statistical analysis revealed hundreds of genes that changed expression by up to 550-fold. The genes responded with varying kinetics suggesting that multiple regulatory pathways are involved. We identified most known oxygen-regulated genes and also uncovered new regulated genes. Reverse transcription-quantitative PCR (RT-qPCR) ana

SUBMITTER: Bendjilali N 

PROVIDER: S-EPMC5217111 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature