Caenorhabditis elegans DAF-2 as a Model for Human Insulin Receptoropathies.

ABSTRACT: Human exome sequencing has dramatically increased the rate of identification of disease-associated polymorphisms. However, examining the functional consequences of those variants has created an analytic bottleneck. Insulin-like signaling in Caenorhabditis elegans has long provided a model to assess consequences of human insulin signaling mutations, but this has not been evaluated in the context of current genetic tools. We have exploited strains derived from the Million Mutation Project (MMP) and gene editing to explore further the evolutionary relationships and conservation between the human and C. elegans insulin receptors. Of 40 MMP alleles analyzed in the C. elegans insulin-like receptor gene DAF-2, 35 exhibited insulin-like signaling indistinguishable from wild-type animals, indicatin

SUBMITTER: Bulger DA 

PROVIDER: S-EPMC5217114 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature