Mass Spectrometric Immunoassays in Characterization of Clinically Significant Proteoforms.

ABSTRACT: Proteins can exist as multiple proteoforms in vivo, as a result of alternative splicing and single-nucleotide polymorphisms (SNPs), as well as posttranslational processing. To address their clinical significance in a context of diagnostic information, proteoforms require a more in-depth analysis. Mass spectrometric immunoassays (MSIA) have been devised for studying structural diversity in human proteins. MSIA enables protein profiling in a simple and high-throughput manner, by combining the selectivity of targeted immunoassays, with the specificity of mass spectrometric detection. MSIA has been used for qualitative and quantitative analysis of single and multiple proteoforms, distinguishing between normal fluctuations and changes related to clinical conditions. This mini review offe

SUBMITTER: Trenchevska O 

PROVIDER: S-EPMC5217360 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature