A Proteomic Study of Clavibacter Michiganensis Subsp. Michiganensis Culture Supernatants.

ABSTRACT: Clavibacter michiganensis, subsp. michiganensis is a Gram-positive plant pathogen infecting tomato (Solanum lycopersicum). Despite a considerable economic importance due to significant losses of infected plants and fruits, knowledge about virulence factors of C. michiganensis subsp. michiganensis and host-pathogen interactions on a molecular level are rather limited. In the study presented here, the proteome of culture supernatants from C. michiganensis subsp. michiganensis NCPPB382 was analyzed. In total, 1872 proteins were identified in M9 and 1766 proteins in xylem mimicking medium. Filtration of supernatants before protein precipitation reduced these to 1276 proteins in M9 and 976 proteins in the xylem mimicking medium culture filtrate.

SUBMITTER: Hiery E 

PROVIDER: S-EPMC5217389 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature