Induction of altered gene expression profiles in cultured bovine granulosa cells at high cell density.

ABSTRACT:

Background

In previous studies it has been shown that bovine granulosa cells (GC) cultured at a high plating density dramatically change their physiological and molecular characteristics, thus resembling an early stage of luteinization. During the present study, these specific effects on the GC transcriptome were comprehensively analysed to clarify the underlying mechanisms.

Methods

GC were cultured in serum free medium with FSH and IGF-1 stimulation at different initial plating density. The estradiol and progesterone production was determined by radioimmunoassays and the gene expression profiles were analysed by mRNA microarray analysis after 9 days. The data were statistically analysed and the abundance of selected, differentially expressed transcripts was re-evaluated by

SUBMITTER: Baufeld A 

PROVIDER: S-EPMC5217602 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature