Evaluation of Different Primers for Detection of Brucella by Using PCR Method.

ABSTRACT:

Introduction

Brucellosis is a worldwide zoonosis and a significant cause of loss of health in humans and animals. Traditionally, classic diagnosis is carried out by isolation of Brucella, which is time-consuming, technically challenging and potentially dangerous. The aim of this study was to expand a molecular test that would be used for the develop detection of Brucella in a single reaction with high sensitivity and specificity, by targeting IS711element.

Methods

This study was carried out from 2015 to 2016 at the Ayatolla Rohani hospital in Babol, Iran. The present study was designed to develop PCR assay, based on IS711 gene for rapid diagnosis of Brucella spp. and immediate detection of Brucella, with high sensitivity and specificity. Four pair

SUBMITTER: Moulana Z 

PROVIDER: S-EPMC5217814 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature