Sulforaphane-induced apoptosis in Xuanwei lung adenocarcinoma cell line XWLC-05.

ABSTRACT:

Background

Xuanwei district in Yunnan Province has the highest incidence of lung cancer in China, especially among non-smoking women. Cruciferous vegetables can reduce lung cancer risk by prompting a protective mechanism against respiratory tract inflammation caused by air pollution, and are rich in sulforaphane, which can induce changes in gene expression. We investigated the effect of sulforaphane-induced apoptosis in Xuanwei lung adenocarcinoma cell line (XWCL-05) to explore the value of sulforaphane in lung cancer prevention and treatment.

Methods

Cell growth inhibition was determined by methyl thiazolyl tetrazolium assay; cell morphology and apoptosis were observed under transmission electron microscope; cell cycle and apoptosis rates were detected using flow cytometry;

SUBMITTER: Zhou L 

PROVIDER: S-EPMC5217876 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature