Marrow-inspired matrix cues rapidly affect early fate decisions of hematopoietic stem and progenitor cells.

ABSTRACT: Hematopoiesis is the physiological process where hematopoietic stem cells (HSCs) continuously generate the body's complement of blood and immune cells within unique regions of the bone marrow termed niches. Although previous investigations have revealed gradients in cellular and extracellular matrix (ECM) content across the marrow, and matrix elasticity and ligand type are believed to be strong regulators of stem cell fate, the impact of biophysical signals on HSC response is poorly understood. Using marrow-inspired ECM ligand-coated polyacrylamide substrates that present defined stiffness and matrix ligand cues, we demonstrate that the interplay between integrin engagement and myosin II activation processes affects the morphology, proliferation, and myeloid lineage specification of primar

SUBMITTER: Choi JS 

PROVIDER: S-EPMC5218514 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature