A bioenergetics assay for studying the effects of environmental stressors on mitochondrial function in vivo in zebrafish larvae.

ABSTRACT: Mitochondria, an integral component of cellular energy metabolism and other key functions, are extremely vulnerable to damage by environmental stressors. Although methods to measure mitochondrial function in vitro exist, sensitive, medium- to high-throughput assays that assess respiration within physiologically-relevant whole organisms are needed to identify drugs and/or chemicals that disrupt mitochondrial function, particularly at sensitive early developmental stages. Consequently, we have developed and optimized an assay to measure mitochondrial bioenergetics in zebrafish larvae using the XF^e24 Extracellular Flux Analyzer. To prevent larval movement from confounding oxygen consumption measurements, we relied on MS-222-based anesthetization. We obtained stable measurement valu

SUBMITTER: Raftery TD 

PROVIDER: S-EPMC5218841 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature