Human Naive Pluripotent Stem Cells Model X Chromosome Dampening and X Inactivation.

ABSTRACT: Naive human embryonic stem cells (hESCs) can be derived from primed hESCs or directly from blastocysts, but their X chromosome state has remained unresolved. Here, we show that the inactive X chromosome (X_i) of primed hESCs was reactivated in naive culture conditions. Like cells of the blastocyst, the resulting naive cells contained two active X chromosomes with XIST expression and chromosome-wide transcriptional dampening and initiated XIST-mediated X inactivation upon differentiation. Both establishment of and exit from the naive state (differentiation) happened via an XIST-negative X_aX_a intermediate. Together, these findings identify a cell culture system for functionally exploring the two X chromosome dosage compensation processes in early human develop

SUBMITTER: Sahakyan A 

PROVIDER: S-EPMC5218861 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature