¹⁵N and ¹³C- SOFAST-HMQC editing enhances 3D-NOESY sensitivity in highly deuterated, selectively [¹H,¹³C]-labeled proteins.

ABSTRACT: The ongoing NMR method development effort strives for high quality multidimensional data with reduced collection time. Here, we apply 'SOFAST-HMQC' to frequency editing in 3D NOESY experiments and demonstrate the sensitivity benefits using highly deuterated and ¹⁵N, methyl labeled samples in H₂O. The experiments benefit from a combination of selective T ₁ relaxation (or L-optimized effect), from Ernst angle optimization and, in certain types of experiments, from using the mixing time for both NOE buildup and magnetization recovery. This effect enhances sensitivity by up to 2.4× at fast pulsing versus reference HMQC sequences of same overall length and water suppression characteristics. Representative experiments designed to address interesting protein NMR c

SUBMITTER: Rossi P 

PROVIDER: S-EPMC5218894 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature