Myostatin signals through miR-34a to regulate Fndc5 expression and browning of white adipocytes.

ABSTRACT:

Background/objectives

Myostatin (Mstn) has a pivotal role in glucose and lipid metabolism. Mstn deficiency leads to the increased browning of white adipose tissue (WAT), which results in the increased energy expenditure and protection against diet-induced obesity and insulin resistance. In this study, we investigated the molecular mechanism(s) through which Mstn regulates browning of white adipocytes.

Methods

Quantitative molecular analyses were performed to assess Mstn regulation of miR-34a and Fndc5 expression. miR-34a was overexpressed and repressed to investigate miR-34a regulation of Fndc5. Luciferase reporter analysis verified direct binding between miR-34a and the Fndc5 3'-untranslated region (UTR). The browning phenotype of Mstn^-/- adipocytes was assessed

SUBMITTER: Ge X 

PROVIDER: S-EPMC5220162 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature