Identification of inhibitors of auxin transcriptional activation by means of chemical genetics in Arabidopsis.

ABSTRACT: Auxin modulates diverse plant developmental pathways through direct transcriptional regulation and cooperative signaling with other plant hormones. Genetic and biochemical approaches have clarified several aspects of the auxin-regulated networks; however, the mechanisms of perception and subsequent signaling events remain largely uncharacterized. To elucidate unidentified intermediates, we have developed a high-throughput screen for identifying small molecule inhibitors of auxin signaling in Arabidopsis. Analysis of 10,000 compounds revealed several potent lead structures that abrogate transcription of an auxin-inducible reporter gene. Three compounds were found to interfere with auxin-regulated proteolysis of an auxin/indole-3-acetic acid transcription factor, and two impart phenotypes in

SUBMITTER: Armstrong JI 

PROVIDER: S-EPMC522024 | biostudies-literature | 2004 Oct

REPOSITORIES: biostudies-literature