Development of a Recombinase Polymerase Amplification Assay for Detection of Epidemic Human Noroviruses.

ABSTRACT: Human norovirus is a leading cause of viral gastroenteritis worldwide. Rapid detection could facilitate control, however widespread point-of-care testing is infrequently done due to the lack of robust and portable methods. Recombinase polymerase amplification (RPA) is a novel isothermal method which rapidly amplifies and detects nucleic acids using a simple device in near real-time. An RT-RPA assay targeting a recent epidemic human norovirus strain (GII.4 New Orleans) was developed and evaluated in this study. The assay successfully detected purified norovirus RNA from multiple patient outbreak isolates and had a limit of detection of 3.40 ± 0.20 log₁₀ genomic copies (LGC), which is comparable to most other reported isothermal norovirus amplification methods. The assay also dete

SUBMITTER: Moore MD 

PROVIDER: S-EPMC5220337 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature