Fermentable carbohydrate stimulates FFAR2-dependent colonic PYY cell expansion to increase satiety.

ABSTRACT:

Objective

Dietary supplementation with fermentable carbohydrate protects against body weight gain. Fermentation by the resident gut microbiota produces short-chain fatty acids, which act at free fatty acid receptor 2 (FFAR2). Our aim was to test the hypothesis that FFAR2 is important in regulating the beneficial effects of fermentable carbohydrate on body weight and to understand the role of gut hormones PYY and GLP-1.

Methods

Wild-type or Ffar2^-/- mice were fed an inulin supplemented or control diet. Mice were metabolically characterized and gut hormone concentrations, enteroendocrine cell density measurements were carried out. Intestinal organoids and colonic cultures were utilized to substantiate the in vivo findings.

Results

We prov

SUBMITTER: Brooks L 

PROVIDER: S-EPMC5220466 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature