Concordance between PIK3CA mutations in endoscopic biopsy and surgically resected specimens of esophageal squamous cell carcinoma.

ABSTRACT:

Background

PIK3CA mutations are expected to be potential therapeutic targets for esophageal squamous cell carcinoma (ESCC). We aimed to clarify the concordance between PIK3CA mutations detected in endoscopic biopsy specimens and corresponding surgically resected specimens.

Methods

We examined five hotspot mutations in the PIK3CA gene (E542K, E545K, E546K, H1047R, and H1047L) in formalin-fixed and paraffin-embedded tissue sections of paired endoscopic biopsy and surgically resected specimens from 181 patients undergoing curative resection for ESCC between 2000 and 2011 using a Luminex technology-based multiplex gene mutation detection kit.

Results

Mutation analyses were successfully performed for both endoscopic biopsy and surgically resected specimens in all the cases

SUBMITTER: Hatogai K 

PROVIDER: S-EPMC5220610 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature