Plasma proteome and metabolome characterization of an experimental human thyrotoxicosis model.

ABSTRACT:

Background

Determinations of thyrotropin (TSH) and free thyroxine (FT₄) represent the gold standard in evaluation of thyroid function. To screen for novel peripheral biomarkers of thyroid function and to characterize FT₄-associated physiological signatures in human plasma we used an untargeted OMICS approach in a thyrotoxicosis model.

Methods

A sample of 16 healthy young men were treated with levothyroxine for 8 weeks and plasma was sampled before the intake was started as well as at two points during treatment and after its completion, respectively. Mass spectrometry-derived metabolite and protein levels were related to FT₄ serum concentrations using mixed-effect linear regression models in a robust setting. To compile a molecular signature

SUBMITTER: Pietzner M 

PROVIDER: S-EPMC5220622 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature