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Repressor mutant forms of the Azospirillum brasilense NtrC protein.

ABSTRACT: The Azospirillum brasilense mutant strains FP8 and FP9, after treatment with nitrosoguanidine, showed a null Nif phenotype and were unable to use nitrate as their sole nitrogen source. Sequencing of the ntrC genes revealed single nucleotide mutations in the NtrC nucleotide-binding site. The phenotypes of these strains are discussed in relation to their genotypes.

SUBMITTER: Huergo LF 

PROVIDER: S-EPMC522079 | biostudies-literature | 2004 Oct

REPOSITORIES: biostudies-literature

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Repressor mutant forms of the Azospirillum brasilense NtrC protein.

Huergo Luciano F LF   Assumpção Marcelo C MC   Souza Emanuel M EM   Steffens M Berenice R MB   Yates M Geoffrey MG   Chubatsu Leda S LS   Pedrosa Fábio O FO  

Applied and environmental microbiology 20041001 10

The Azospirillum brasilense mutant strains FP8 and FP9, after treatment with nitrosoguanidine, showed a null Nif phenotype and were unable to use nitrate as their sole nitrogen source. Sequencing of the ntrC genes revealed single nucleotide mutations in the NtrC nucleotide-binding site. The phenotypes of these strains are discussed in relation to their genotypes. ...[more]

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