Novel CagA ELISA exhibits enhanced sensitivity of Helicobacter pylori CagA antibody.

ABSTRACT:

Aim

To develop a novel Helicobacter pylori (H. pylori) CagA antibody enzyme-linked immunosorbent assay (ELISA) suitable for detecting serum anti-CagA antibodies with high sensitivity.

Methods

Recombinant East Asian-type CagA protein was purified and immobilized for ELISA. Serum samples from 217 Vietnamese individuals (110 H. pylori-infected and 107 uninfected individuals) were applied. Conventional ELISA from Western-type CagA and our East Asian-type CagA ELISA were evaluated by comparing 38 subjects with the Western-type genotype and 72 subjects with the East Asian-type cagA genotype. Histological scores of the gastric mucosa were determined using the updated Sydney System to examine the relationship with anti-CagA antibody titers.

Results

SUBMITTER: Matsuo Y 

PROVIDER: S-EPMC5221286 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature