Identification of the vascular endothelial growth factor signalling pathway by quantitative proteomic analysis of rat condylar cartilage.

ABSTRACT: Angiogenesis mediated by vascular endothelial growth factor (VEGF) is known to play an important role in regulating cartilage remodelling and endochondral ossification. However, the details of how VEGF signalling mechanisms affect condyle remodelling in response to alterations in functional loading remains unclear. To explore this, eighty 16-day-old male SD rats were divided into two equal groups which were fed either a soft/powdery diet or a hard diet for 4 weeks; the stiffness of the diet results in alteration of mastication force and hence temporomandibular joint (TMJ) development. We performed a proteomic analysis of rat condylar cartilage using isobaric tags for relative and absolute quantification (iTRAQ) labelling, followed by 2D nano-high performance liquid chromatography and MALDI

SUBMITTER: Jiang L 

PROVIDER: S-EPMC5221432 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature