Live-cell single-molecule labeling and analysis of myosin motors with quantum dots.

ABSTRACT: Quantum dots (QDs) are a powerful tool for quantitatively analyzing dynamic cellular processes by single-particle tracking. However, tracking of intracellular molecules with QDs is limited by their inability to penetrate the plasma membrane and bind to specific molecules of interest. Although several techniques for overcoming these problems have been proposed, they are either complicated or inconvenient. To address this issue, in this study, we developed a simple, convenient, and nontoxic method for labeling intracellular molecules in cells using HaloTag technology and electroporation. We labeled intracellular myosin motors with this approach and tracked their movement within cells. By simultaneously imaging myosin movement and F-actin architecture, we observed that F-actin serves not only

SUBMITTER: Hatakeyama H 

PROVIDER: S-EPMC5221621 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature