The Escherichia coli dnaN159 mutant displays altered DNA polymerase usage and chronic SOS induction.

ABSTRACT: The Escherichia coli beta sliding clamp, which is encoded by the dnaN gene, is reported to interact with a variety of proteins involved in different aspects of DNA metabolism. Recent findings indicate that many of these partner proteins interact with a common surface on the beta clamp, suggesting that competition between these partners for binding to the clamp might help to coordinate both the nature and order of the events that take place at a replication fork. The purpose of the experiments discussed in this report was to test a prediction of this model, namely, that a mutant beta clamp protein impaired for interactions with the replicative DNA polymerase (polymerase III [Pol III]) would likewise have impaired interactions with other partner proteins and hence would display pleiotropic p

SUBMITTER: Sutton MD 

PROVIDER: S-EPMC522196 | biostudies-literature | 2004 Oct

REPOSITORIES: biostudies-literature