The Ciliopathy-Associated Cep104 Protein Interacts with Tubulin and Nek1 Kinase.

ABSTRACT: Cilia are thin cell projections with essential roles in cell motility, fluid movement, sensing, and signaling. They are templated from centrioles that dock against the plasma membrane and subsequently extend their peripheral microtubule array. The molecular mechanisms underpinning cilia assembly are incompletely understood. Cep104 is a key factor involved in cilia formation and length regulation that rides on the ends of elongating and shrinking cilia. It is mutated in Joubert syndrome, a genetically heterogeneous ciliopathy. Here we provide structural and biochemical data that Cep104 contains a tubulin-binding TOG (tumor overexpressed gene) domain and a novel C2HC zinc finger array. Furthermore, we identify the kinase Nek1, another ciliopathy-associated protein, as a potential binding par

SUBMITTER: Al-Jassar C 

PROVIDER: S-EPMC5222566 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature