High fat diet-induced changes of mouse hepatic transcription and enhancer activity can be reversed by subsequent weight loss.

ABSTRACT: Epigenetic factors have been suggested to play an important role in metabolic memory by trapping and maintaining initial metabolic changes within the transcriptional regulatory machinery. In this study we fed mice a high fat diet (HFD) for seven weeks followed by additional five weeks of chow, to identify HFD-mediated changes to the hepatic transcriptional program that may persist after weight loss. Mice fed a HFD displayed increased fasting insulin levels, hepatosteatosis and major changes in hepatic gene transcription associated with modulation of H3K27Ac at enhancers, but no significant changes in chromatin accessibility, indicating that HFD-regulated gene transcription is primarily controlled by modulating the activity of pre-established enhancers. After return to the same body weight

SUBMITTER: Siersbæk M 

PROVIDER: S-EPMC5223143 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature