Cell Painting, a high-content image-based assay for morphological profiling using multiplexed fluorescent dyes.

ABSTRACT: In morphological profiling, quantitative data are extracted from microscopy images of cells to identify biologically relevant similarities and differences among samples based on these profiles. This protocol describes the design and execution of experiments using Cell Painting, which is a morphological profiling assay that multiplexes six fluorescent dyes, imaged in five channels, to reveal eight broadly relevant cellular components or organelles. Cells are plated in multiwell plates, perturbed with the treatments to be tested, stained, fixed, and imaged on a high-throughput microscope. Next, an automated image analysis software identifies individual cells and measures ∼1,500 morphological features (various measures of size, shape, texture, intensity, and so on) to produce a rich profile t

SUBMITTER: Bray MA 

PROVIDER: S-EPMC5223290 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature