Protein-crystal interface mediates cell adhesion and proangiogenic secretion.

ABSTRACT: The nanoscale materials properties of bone apatite crystals have been implicated in breast cancer bone metastasis and their interactions with extracellular matrix proteins are likely involved. In this study, we used geologic hydroxyapatite (HAP, Ca₁₀(PO₄)₆(OH)₂), closely related to bone apatite, to investigate how HAP surface chemistry and nano/microscale topography individually influence the crystal-protein interface, and how the altered protein deposition impacts subsequent breast cancer cell activities. We first utilized Förster resonance energy transfer (FRET) to assess the molecular conformation of fibronectin (Fn), a major extracellular matrix protein upregulated in cancer, when it adsorbed onto HAP facets. Our analysis reveals that both lo

SUBMITTER: Wu F 

PROVIDER: S-EPMC5223748 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature