Persistence to anti-cancer treatments in the stationary to proliferating transition.

ABSTRACT: The heterogeneous responses of clonal cancer cells to treatment is understood to be caused by several factors, including stochasticity, cell-cycle dynamics, and different micro-environments. In a tumor, cancer cells may encounter fluctuating conditions and transit from a stationary culture to a proliferating state, for example this may occur following treatment. Here, we undertake a quantitative evaluation of the response of single cancerous lymphoblasts (L1210 cells) to various treatments administered during this transition. Additionally, we developed an experimental system, a "Mammalian Mother Machine," that tracks the fate of thousands of mammalian cells over several generations under transient exposure to chemotherapeutic drugs. Using our developed system, we were able to follow the sa

SUBMITTER: Pearl Mizrahi S 

PROVIDER: S-EPMC5224467 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature