Fingerprinting of neurotoxic compounds using a mouse embryonic stem cell dual luminescence reporter assay.

ABSTRACT: Identification of neurotoxic drugs and environmental chemicals is an important challenge. However, only few tools to address this topic are available. The aim of this study was to develop a neurotoxicity/developmental neurotoxicity (DNT) test system, using the pluripotent mouse embryonic stem cell line CGR8 (ESCs). The test system uses ESCs at two differentiation stages: undifferentiated ESCs and ESC-derived neurons. Under each condition, concentration-response curves were obtained for three parameters: activity of the tubulin alpha 1 promoter (typically activated in early neurons), activity of the elongation factor 1 alpha promoter (active in all cells), and total DNA content (proportional to the number of surviving cells). We tested 37 compounds from the ESNATS test battery, which includ

SUBMITTER: Colaianna M 

PROVIDER: S-EPMC5225183 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature