Distinct 5-methylcytosine profiles in poly(A) RNA from mouse embryonic stem cells and brain.

ABSTRACT:

Background

Recent work has identified and mapped a range of posttranscriptional modifications in mRNA, including methylation of the N6 and N1 positions in adenine, pseudouridylation, and methylation of carbon 5 in cytosine (m5C). However, knowledge about the prevalence and transcriptome-wide distribution of m5C is still extremely limited; thus, studies in different cell types, tissues, and organisms are needed to gain insight into possible functions of this modification and implications for other regulatory processes.

Results

We have carried out an unbiased global analysis of m5C in total and nuclear poly(A) RNA of mouse embryonic stem cells and murine brain. We show that there are intriguing differences in these samples and cell compartments with respect to the degree of me

SUBMITTER: Amort T 

PROVIDER: S-EPMC5225599 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature