In vivo imaging of skeletal muscle in mice highlights muscle defects in a model of myotubular myopathy.

ABSTRACT: Skeletal muscle structure and function are altered in different myopathies. However, the understanding of the molecular and cellular mechanisms mainly rely on in vitro and ex vivo investigations in mammalian models. In order to monitor in vivo the intracellular structure of the neuromuscular system in its environment under normal and pathological conditions, we set-up and validated non-invasive imaging of ear and leg muscles in mice. This original approach allows simultaneous imaging of different cellular and intracellular structures such as neuromuscular junctions and sarcomeres, reconstruction of the 3D architecture of the neuromuscular system, and video recording of dynamic events such as spontaneous muscle fiber contraction. Second harmonic generation was combined

SUBMITTER: Mercier L 

PROVIDER: S-EPMC5226009 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature