Identification of Cryptococcus neoformans temperature-regulated genes with a genomic-DNA microarray.

ABSTRACT: The ability to survive and proliferate at 37 degrees C is an essential virulence attribute of pathogenic microorganisms. A partial-genome microarray was used to profile gene expression in the human-pathogenic fungus Cryptococcus neoformans during growth at 37 degrees C. Genes with orthologs involved in stress responses were induced during growth at 37 degrees C, suggesting that a conserved transcriptional program is used by C. neoformans to alter gene expression during stressful conditions. A gene encoding the transcription factor homolog Mga2 was induced at 37 degrees C and found to be important for high-temperature growth. Genes encoding fatty acid biosynthetic enzymes were identified as potential targets of Mga2, suggesting that membrane remodeling is an important component of adaptatio

SUBMITTER: Kraus PR 

PROVIDER: S-EPMC522612 | biostudies-literature | 2004 Oct

REPOSITORIES: biostudies-literature