Directed Protein Packaging within Outer Membrane Vesicles from Escherichia coli: Design, Production and Purification.

ABSTRACT: An increasing interest in applying synthetic biology techniques to program outer membrane vesicles (OMV) are leading to some very interesting and unique applications for OMV where traditional nanoparticles are proving too difficult to synthesize. To date, all Gram-negative bacteria have been shown to produce OMV demonstrating packaging of a variety of cargo that includes small molecules, peptides, proteins and genetic material. Based on their diverse cargo, OMV are implicated in many biological processes ranging from cell-cell communication to gene transfer and delivery of virulence factors depending upon which bacteria are producing the OMV. Only recently have bacterial OMV become accessible for use across a wide range of applications through the development of techniques to control and d

SUBMITTER: Alves NJ 

PROVIDER: S-EPMC5226233 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature