Inferential Structure Determination of Chromosomes from Single-Cell Hi-C Data.

ABSTRACT: Chromosome conformation capture (3C) techniques have revealed many fascinating insights into the spatial organization of genomes. 3C methods typically provide information about chromosomal contacts in a large population of cells, which makes it difficult to draw conclusions about the three-dimensional organization of genomes in individual cells. Recently it became possible to study single cells with Hi-C, a genome-wide 3C variant, demonstrating a high cell-to-cell variability of genome organization. In principle, restraint-based modeling should allow us to infer the 3D structure of chromosomes from single-cell contact data, but suffers from the sparsity and low resolution of chromosomal contacts. To address these challenges, we adapt the Bayesian Inferential Structure Determination (ISD) f

SUBMITTER: Carstens S 

PROVIDER: S-EPMC5226817 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature